Vitrification of immature bubaline cumulus oocyte complexes by the open-pulled straw and conventional straw methods and their subsequent in vitro fertilization
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منابع مشابه
Vitrification of immature bubaline cumulus oocyte complexes by the open-pulled straw and conventional straw methods and their subsequent in vitro fertilization
The in vitro maturation (IVM), fertilization (IVF) and morphological changes in buffalo cumulus oocyte complexes (COCs) cryopreserved by ultra rapid freezing using conventional (CON) and open-pulled straw (OPS) methods were tested. COCs were cryopreserved using a vitrification solution comprising of DPBS + 0.5M sucrose + 0.4% BSA and two concentrations (4.5 or 5.5M) of each cryoprotectant ethyl...
متن کاملThe Survival Rate, Fertilization and Developmental Capacity of Mature Mouse Oocyte Which Vitrified in Open Pulled Straw
Purpose: The aim of this study was to evaluate the effect of vitrification in open pulled straw on survival rate, fertilization and development capacity and comparison with conventional straw. Materials and Methods: NMRI mice were used for retrieval of oocytes. After administration of HMG and HCG, the mice scarified by cervical dislocation. Mice's abdomens were dissected and mature oocytes wer...
متن کاملEffect of vitrification on number of inner cell mass in mouse blastocysts in conventional straw, closed pulled straw, open pulled straw and cryoloop carriers.
OBJECTIVE To compare the effect of using open and closed carriers on count of inner cell mass in vitrified mouse blastocyst after warming. METHODS The experimental study was conducted at Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, from April to September 2010. Forty female NMRI (Naval Medical Research Institute, USA) mice were injected with pregnant mare's serum gonadotropi...
متن کاملCryopreservation of Rat Bone Marrow Derived Mesenchymal Stem Cells by Two Conventional and Open-Pulled Straw Vitrification Methods
Bone marrow (BM) is a complex tissue containing populations of progenitor and stem cells (1). One type, hematopoietic stem cells (HSCs), can renew circulating blood elements such as red blood cells, monocytes, platelets, granulocytes and lymphocytes. The other is mesenchymal stem cells (MSCs), which possess two important properties of long-term self renewal and differentiate into osteoblasts, c...
متن کاملeffects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization
background: vitrification has proven to be more effective than slow freezing methods to cryopreserve mammalian oocytes. the objectives of this study were to evaluate the effects of vitrification on immature and in vitro matured, denuded and cumulus compact goat oocytes and their subsequent fertilization. methods: oocytes were either cryopreserved as immature cumulus compact (imcc) (n=98 exp 1; ...
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ژورنال
عنوان ژورنال: Veterinární Medicína
سال: 2008
ISSN: 0375-8427,1805-9392
DOI: 10.17221/1928-vetmed